一、13 CODIS Core STR Loci
2000年,FBI公布了13对CODIS (Combined DNA Index System)Core STR Loci,如图1:
STR位点信息:
Loci Name | Gene Name | Chromosomal Location | Primer | Primer # | Size Range | Repeat Structure | Mutation Rate |
---|---|---|---|---|---|---|---|
TPOX | TPO | 2p25.3 | GCACAGAACAGGCACTTAGG CGCTCAAACGTGAGGTTG | #5 | 254-302 | [AATG]n | 0.01% |
D3S1358 | LARS2 | 3p21.31 | ACTGCAGTCCAATCTGGGT ATGAAATCAACAGAGGCTTGC | #3 | 99-147 | TCTA[TCTG]n[TCTA]n | 0.12% |
FGA | 4q28 | GGCTGCAGGGCATAACATTA ATTCTATGACTTTGCGCTTCAGGA | #3 | 308-464 | [TTTC]3TTTTTTCT[CTTT]nCTCC[TTCC]2 | 0.28% | |
CSF1PO | CSF1R | 5q33.1 | AACCTGAGTCTGCCAAGGACTAGC TTCCACACACCACTGGCCATCTTC | #1 | 287-331 | [AGAT]n | 0.16% |
D5S818 | 5q23.2 | GGGTGATTTTCCTCTTTGGT TGATTCCAATCATAGCCACA | #2 | 129-177 | [AGAT]n | 0.11% | |
D7S820 | 7q21.11 | ATGTTGGTCAGGCTGACTATG GATTCCACATTTATCCTCATTGAC | #1 | 211-251 | [GATA]n | 0.10% | |
D8S1179 | 8q24.13 | ATTGCAACTTATATGTATTTTTGTATTTCATG ACCAAATTGTGTTCATGAGTATAGTTTC | #3 | 203-255 | [TCTA]n[TCTG]n[TCTA]n | 0.14% | |
TH01 | TH | 11p15.5 | GTGGGCTGAAAAGCTCCCGATTAT ATTCAAAGGGTATCTGGGCTCTGG | #1 | 171-215 | [AATG]nATG[AATG]n | 0.01% |
VWA | VWF | 12p13.31 | GCCCTAGTGGATGATAAGAATAATCAGTATGTG GGACAGATGATAAATACATAGGATGGATGG | #2 | 123-183 | [TCTA][TCTG]n[TCTA]n[TCCA][TCTA] | 0.17% |
D13S317 | 13q31.1 | ATTACAGAAGTCTGGGATGTGGAGGA GGCAGCCCAAAAAGACAGA | #1 | 157-205 | [TATC]n[AATC]n | 0.14% | |
D16S539 | 16q24.1 | GGGGGTCTAAGAGCTTGTAAAAAG GTTTGTGTGTGCATCTGTAAGCATGTATC | #1 | 260-308 | [GATA]n | 0.11% | |
D18S51 | 18q21.33 | TTCTTGAGCCCAGAAGGTTA ATTCTACCAGCAACAACACAAATAAAC | #3 | 286-373 | [AGAA]n(AAAGAGAGAG) | 0.22% | |
D21S11 | 21q21.1 | ATATGTGAGTCAATTCCCCAAG TGTATTAGTCAATGTTCTCCAGAGAC | #4 | 155-273 | [TCTA]n[TCTG]n[TCTA]n[TA][TCTA]n[TCA][TCTA]n[TCCATA][TCTA]n | 0.19% | |
AMELX | X | CCCTGGGCTCTGTAAAGAA ATCAGAGCTTAAACTGGGAAGCTG | #1 | 106 | - | ||
AMELY | Y | CCCTGGGCTCTGTAAAGAA ATCAGAGCTTAAACTGGGAAGCTG | #1 | 112 | - |
二、Additional 7 STR loci
从2017年1月1日起,对于上传至National DNA Index System (NDIS)的数据,FBI要求增加7个STR位点,如下:
Loci Name | Gene Name | Chromosomal Location | Primer | Primer # | Size Range | Repeat Structure | Mutation Rate |
---|---|---|---|---|---|---|---|
D1S1656 | 1q42 | GTGTTGCTCAAGGGTCAACT GAGAAATAGAATCACTAGGGAACC | #1 | 121-169 | [TAGA]n[TGA]n[TAGA]n[TAGG]n[TG]n | ||
D2S441 | 2p14 | TGCACCCAACATTCTAACAA ATTGGAGCTAAGTGGCTGTG | #1 | 78-114 | [TCTA]n | ||
D2S1338 | 2q35 | [TGCC]n[TTCC]n | 0.12% | ||||
D10S1248 | 10q26.3 | GGAATAAGTGCAGTGCTTGG ACCAATCTGGTCACAACCAT | #1 | 227-271 | [GGAA]n | ||
D12S391 | Chr12 | AACAGGATCAATGGATGCAT TGGCTTTTAGACCTGGACTG | #1 | 209-253 | [AGAT]8-17[AGAC]6-10[AGAT]0-1 | ||
D19S433 | 19q12 | CCTGGGCAACAGAATAAGAT TAGGTTTTTAAGGAACAGGTGG | #1 | [AAGG][AAAG][AAGG][TAGG][AAGG]12 | 0.11% | ||
D22S1045 | IL2RB | 22q12.3 | GCTAGATTTTCCCCGATGAT ATGTAAAGTGCTCTCAAGAGTGC | #1 | 129-165 | [ATT]n[ACT][ATT]n |